FastQCFastQC Report
Sat 18 Jun 2016
SRR3137988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568820
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28500.035.9625661
GTATCAA53400.033.1524121
ATCAACG63350.026.4671363
CAACGCA63750.026.3548185
TCAACGC65850.025.5143454
TATCAAC70000.024.6920452
AACGCAG69050.024.5813986
TATGCCG11350.023.71015243
TGCGACG3950.023.58152422
TCTCGTA11400.021.7890838
CGACGAG4300.021.66209624
TCGTATG12750.021.10609240
ACGCAGA80350.020.7373987
CTCGTAT12050.020.61431339
ACGTGCG2700.020.4432149
CAATGCG4750.020.33561919
CGCAGAG82150.020.1150428
ATGCGAC5650.019.53919621
GCTCGAT4600.019.49992264
AATGCGA5250.019.05599820
Produced by FastQC (version 0.11.5)