FastQCFastQC Report
Sat 18 Jun 2016
SRR3137987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672114
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168250.45818294312213614No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG41070.11184293298083883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.041.5218351
GTATCAA106150.036.3724821
GCTCGAT10150.036.04755464
CTCGATA11450.031.95875265
TGGTCGT11450.030.74351551
CGACGAG7300.029.77012824
ATCAACG131950.028.7195223
GCTCGTC12750.028.69079839
CAACGCA134100.028.5747555
TATCAAC136400.028.2053872
GTCGTCT12750.028.15061653
CGATAAT13400.027.05152167
TCAACGC141900.026.7571954
AACGCAG144750.026.738616
TCGATAA14250.025.92174766
CTGGTCG14000.025.88332750
CATTGCG14500.025.69920229
GTCAGCG14600.025.53608943
TCAGCGC14500.025.4730844
CAATGCG8550.025.4132619
Produced by FastQC (version 0.11.5)