FastQCFastQC Report
Sat 18 Jun 2016
SRR3137987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672114
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG47880.13038810886590121No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG40870.11129828758039648TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47000.040.827511
GTATCAA100000.037.2044331
CGACGAG8750.031.93562924
TATGCCG17100.030.867843
GCTCGAT9400.030.09554964
ATCAACG128000.028.4915963
CAATGCG10050.028.14791919
CAACGCA131250.028.0748735
TATCAAC133800.027.8234632
CTCGTAT17000.027.59982339
TGGTCGT10350.027.33352751
ATCTCGT17450.027.08541737
GATCTCG17500.027.00802636
TCGTATG19400.026.85291940
ACGTGCG5600.026.4898179
AACGCAG141150.026.4713236
CTCGATA10850.026.39153365
TCAACGC139650.026.287694
TCTCGTA18050.026.18506838
GCGCGAT5850.025.94748713
Produced by FastQC (version 0.11.5)