FastQCFastQC Report
Sat 18 Jun 2016
SRR3137986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648600
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103350.3902061466435098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.034.432741
GTATCAA47800.032.7759321
ATCAACG57050.026.6242663
CAACGCA58750.026.5087685
TCAACGC60700.025.3122794
TATCAAC61350.025.0346262
AACGCAG63700.024.8302766
TGCGACG3600.022.0353522
GCTCGAT4050.021.3071464
GTACTAG9150.021.0735871
ACGCAGA74350.020.9087057
CGCAGAG75450.020.1934748
CGACGAG4150.019.94835724
CTCGATA4450.019.39409665
TCGTCTA4600.018.75919254
AGAGTAC77050.018.75049411
GTACATG62150.018.4490761
GCAGAGT83200.017.8998559
GATCGCA4250.017.84694710
CAGAGTA82050.017.816310
Produced by FastQC (version 0.11.5)