FastQCFastQC Report
Sat 18 Jun 2016
SRR3137986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648600
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.038.2894131
GTATCAA49000.033.1085321
ATCAACG56750.027.420063
CAACGCA57700.027.0876855
TGCGACG4750.026.8730322
AACGCAG60450.026.0256546
TATCAAC61850.025.9968242
TCAACGC60600.025.8483454
CAATGCG4700.025.69037819
CGACGAG4750.025.41995424
TATGCCG7150.025.0907743
GCTCGAT4200.024.64318764
ACGATCG1851.8189894E-1124.2431160
CGTACGA3150.024.09510660
GCGAATC9900.023.69728733
AATCTCG8250.022.58169636
ACGCAGA69350.022.3854797
AATCGCG1253.5062549E-622.0798836
CGCGATA2207.2759576E-1221.95318214
TAAGGCG12450.021.8918629
Produced by FastQC (version 0.11.5)