FastQCFastQC Report
Sat 18 Jun 2016
SRR3137985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177856
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60210.1894673641599871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.039.1270031
GTATCAA62950.034.363061
ATCAACG74400.028.387363
CAACGCA76050.028.1911965
TCAACGC75750.027.9785884
TATCAAC77200.027.306682
AACGCAG81700.026.4563246
CTCGATA5350.024.51748365
TGCGACG4200.023.81620422
ACGCAGA92050.022.9234587
GCTCGAT6100.022.63406264
CGCAGAG94650.022.0758888
ACGTGCG2101.03682396E-1021.3450459
TCGTCTA6400.021.03210354
CATTGCG7000.020.70003329
GAGTACT61450.020.6512812
AGAGTAC97150.020.48113611
CGATAAT6800.020.30536867
GCAGAGT103850.019.7552979
GCGCGAT2102.3665052E-919.70591713
Produced by FastQC (version 0.11.5)