FastQCFastQC Report
Sat 18 Jun 2016
SRR3137985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177856
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.041.3628271
GTATCAA56150.032.9443931
GCTCGAT4550.027.29737764
CGACGAG4500.026.83314924
ATCAACG67050.026.6024443
CTCGATA4800.026.59440465
CAACGCA67900.026.3198245
TCAACGC69350.025.8192654
TGCGACG4850.025.60726522
TATCAAC71300.025.5494172
CAATGCG5150.024.7850719
AACGCAG72750.024.7070476
CGCACTA5050.024.59466458
ATGCGAC6050.022.80904421
GCGCGAT2155.456968E-1222.46354913
TCGTCTA6500.022.29250754
CGATAAT6100.021.4926767
GTCGTCT6100.021.49199353
GTACTAG9000.021.4739531
GTGTAGC18600.020.7812461
Produced by FastQC (version 0.11.5)