FastQCFastQC Report
Sat 18 Jun 2016
SRR3137984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4748095
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135610.28560928119593226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.037.261431
GTATCAA76000.031.9940451
ATCAACG90500.025.9597743
CAACGCA92750.025.601085
TCAACGC93950.025.1207284
TATCAAC98100.024.4683782
AACGCAG98300.024.370636
GCTCGAT5500.021.33840264
CTCGATA5650.020.77189465
ACGCAGA114850.020.4131347
CGCAGAG116200.020.1172738
GTGTAGC24550.019.6405931
GTACATG112900.019.4322721
TACATGG113050.018.765132
AGAGTAC120950.018.13321111
ACATGGG114500.017.9912263
TGCGACG4850.017.78075422
TCGTCTA6800.017.76492954
CATTGCG7350.017.36723129
CAGAGTA130550.017.19437210
Produced by FastQC (version 0.11.5)