FastQCFastQC Report
Sat 18 Jun 2016
SRR3137984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4748095
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40850.037.8472941
GTATCAA78500.033.5431481
ATCAACG94000.027.5267073
TCAACGC96000.027.0969834
CAACGCA95800.026.9737745
TATGCCG13000.026.00779543
TATCAAC100750.025.922462
AACGCAG101950.025.4137616
CTCGTAT12100.025.09074639
TCTCGTA12400.024.48371138
CTCGATA6750.022.48992565
TCGTATG14850.022.30288340
ACGCAGA117150.021.8789357
CGTACGA3350.021.62763660
TGCGACG4950.021.60523622
CGCAGAG119500.021.2466058
ATCTCGT14550.020.86606437
TGCCGTC16750.020.80328845
GCTCGAT7350.020.65401564
GCCGTCT17000.020.49735646
Produced by FastQC (version 0.11.5)