FastQCFastQC Report
Sat 18 Jun 2016
SRR3137983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562369
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162040.35516636203691543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.037.014781
GTATCAA77600.031.6943991
ATCAACG92850.025.9351623
CAACGCA95650.025.5816865
TCAACGC96400.025.1980954
AACGCAG102550.024.1322086
TATCAAC99500.024.0929132
TGCGACG3400.023.33102822
ACGCAGA117050.020.5870957
CGCAGAG118750.020.1195188
CGTACGA2603.6379788E-1219.912860
GTACATG115300.019.1203861
CGATCGG2002.8676368E-818.98562261
CGACGAG4200.018.88681624
TACATGG114650.018.656092
AGAGTAC121500.018.24857511
ACATGGG116800.017.8445743
CAGAGTA128900.017.54755410
GCAGAGT135100.017.0980649
GAGTACT75100.017.08093312
Produced by FastQC (version 0.11.5)