FastQCFastQC Report
Sat 18 Jun 2016
SRR3137983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562369
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.039.481841
TATGCCG16000.031.48130643
GTATCAA79450.030.8446251
TCGTATG17550.028.70059240
CTCGTAT15750.027.8187939
TCTCGTA16350.026.58691238
ATCAACG91400.026.1218263
TCAACGC94450.025.4241334
CAACGCA94200.025.381735
ATGCCGT19850.025.37535944
CCGTCTT19750.025.32915947
TATCAAC99700.024.363262
AACGCAG100400.023.91696
CGTATGC21100.023.8720841
GCCGTCT21150.023.65252346
TGCCGTC21150.023.65252345
ATCTCGT18800.022.93861837
GATCTCG19050.022.81868736
CGTCTTC23000.022.04979748
ACGCAGA115350.020.5169167
Produced by FastQC (version 0.11.5)