FastQCFastQC Report
Sat 18 Jun 2016
SRR3137982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4612423
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291670.6323574398965576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.036.341181
GTATCAA75800.033.2607271
ATCAACG87400.028.4167883
CAACGCA90800.027.7444465
TATCAAC93300.026.7622472
TCAACGC93750.026.7544544
AACGCAG97300.026.1440566
ACGCAGA109900.022.5566147
CGCAGAG112100.021.8991158
CAATGCG6300.021.35330419
TGCGACG6400.020.48158322
AGAGTAC116350.019.97723211
GAGTACT68300.019.18835612
GCAGAGT127400.018.81019
ATGCGAC7750.018.6934121
CAGAGTA125650.018.68972210
ATCGTCA7400.018.64002610
GTACTAG14100.018.3202741
GCTCGAT6250.018.22401864
GTACATG110950.018.1911471
Produced by FastQC (version 0.11.5)