FastQCFastQC Report
Sat 18 Jun 2016
SRR3137982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4612423
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG74050.1605446855156173TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.036.9387631
GTATCAA71400.033.840521
TATGCCG29550.031.05594343
TCTCGTA27300.030.70822738
TGCGACG4650.028.93401122
CTCGTAT29000.028.9080939
TCGTATG32250.028.02769540
ATGCCGT32650.028.00162544
GATCTCG30600.027.73448436
ATCTCGT30650.027.46412537
ATCAACG86450.027.4588073
CAACGCA86500.027.4030445
TGCCGTC34650.026.38536645
TCAACGC90550.026.3298154
TATCAAC91450.026.1472
CGTATGC35000.026.02294341
AACGCAG94000.025.912636
GCTCGAT6700.025.74607564
CCGTCTT36300.025.18603347
CGTCTTC37500.024.56408348
Produced by FastQC (version 0.11.5)