FastQCFastQC Report
Sat 18 Jun 2016
SRR3137981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635231
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44160.16757544215288905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.038.633481
GTATCAA51800.033.9107971
CGATAAT5650.027.49392767
ATCAACG63250.027.4515363
TGCGACG4400.027.43726222
CAACGCA65350.026.8442025
TATCAAC65650.026.4429512
TCAACGC66300.026.2466324
AACGCAG70550.025.015026
CAATGCG5250.024.96271519
CTCGATA6400.024.81088865
ATGCGAC5600.024.6347321
GCTCGAT7250.022.85428664
CGTACGA2600.022.56399360
TCGTCTA7250.022.37346854
ACGCAGA80200.021.5360287
CGACGAG5650.021.36869624
GTGGTAT11650.021.2864931
CGTCTAC7850.020.66300255
CGCAGAG82900.020.585888
Produced by FastQC (version 0.11.5)