FastQCFastQC Report
Sat 18 Jun 2016
SRR3137981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635231
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.039.392781
GTATCAA55450.033.6135941
TGCGACG5750.030.59808722
CAATGCG5800.030.33316419
GTACTAG8900.029.8623181
ATGCGAC6350.029.3367521
CGCACTA6600.028.2255158
CAACGCA67300.027.1220955
ATCAACG67500.027.0417353
CGACGAG6650.026.9757624
TATCAAC69550.026.7407912
TCAACGC69350.026.3701174
AATGCGA6950.025.8103620
AACGCAG72450.025.6675016
GCGACGA7600.023.6037923
CTCGATA5900.023.389765
ATCGTCA8250.022.9976910
GCGCACT8500.022.72799157
CGTAACG1103.2705524E-521.95317623
ACGAGTC8350.021.89808526
Produced by FastQC (version 0.11.5)