FastQCFastQC Report
Sat 18 Jun 2016
SRR3137980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739646
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275691.0062978939614826No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG39530.1442887146733556No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG33550.12246107708806175No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC31490.11494185745165617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46300.043.960111
GTATCAA96950.036.552731
CGACGAG7250.036.1592224
GCTCGAT9450.033.97010864
CAATGCG8450.032.2435119
CTCGATA10400.031.20011365
CGATAAT10200.031.13902767
TGGTCGT10500.030.24324451
GTACTAG10700.029.933081
CTGGTCG10700.029.6768650
TATCACG1650.029.2210852
TCGTCTA11050.028.42295354
ATCGTCA9750.028.29085210
AGTCCTA9750.027.59873429
GTCGTCT11800.027.20238153
GTCTACC11700.027.13689856
ATCAACG127400.027.0942763
CAACGCA130450.026.787985
TATCAAC132900.026.6909792
TCGATAA12100.026.532866
Produced by FastQC (version 0.11.5)