FastQCFastQC Report
Sat 18 Jun 2016
SRR3137979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712599
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190310.7015780806525402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.037.8696251
GTATCAA50000.032.4419251
ATCAACG57000.027.498723
CAACGCA58050.027.1260285
TCAACGC60550.025.8315074
TATCAAC63150.025.3619942
AACGCAG62950.025.1857386
CGACGAG3850.024.19251624
TGCGACG4000.023.28443322
ACGCAGA69750.022.4878587
CGCAGAG70850.021.8970538
CGCACTA4650.021.5252458
AGAGTAC72900.020.05578411
CAATGCG4650.020.02406719
GAGTACT50450.019.82450712
CGGGATA3000.019.55936450
CAGAGTA77700.019.0816810
GTACATG58250.018.6238861
ATGCGAC5200.018.57207321
GCAGAGT81900.018.4796459
Produced by FastQC (version 0.11.5)