FastQCFastQC Report
Sat 18 Jun 2016
SRR3137979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712599
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG46030.16968965925298948RNA PCR Primer, Index 27 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG28320.1044017195317111RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.039.746791
GTATCAA46850.032.9329261
TATGCCG18350.031.77362843
TCTCGTA18300.031.29487238
CTCGTAT18000.031.24145739
TCGTATG19800.029.09829140
ATCTCGT20550.028.03630837
TATCTCG20200.028.00971436
ATGCCGT20950.027.5010144
CAACGCA55050.027.325585
ATCAACG55000.027.1622283
GCCGTCT21650.025.9744246
TGCCGTC21700.025.91457445
CCGTCTT22050.025.81615447
CTCGATA4150.025.77091265
TATCAAC59350.025.5783272
TCAACGC59000.025.4961554
GTATGCC23200.025.13129642
CGTATGC23100.025.09074241
AACGCAG60700.025.065836
Produced by FastQC (version 0.11.5)