FastQCFastQC Report
Sat 18 Jun 2016
SRR3137978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989000
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142900.4780863164938106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.041.101091
GTATCAA69450.035.1622471
GCTCGAT7050.030.35295964
CGACGAG4900.030.2712524
ATCAACG84950.027.8629423
CAACGCA88600.027.3070815
CTCGATA7900.027.08986565
TGCGACG5500.026.96847722
TATCAAC90450.026.5810012
AACGCAG93950.026.1591076
TCAACGC92700.025.7591084
GCGCGAT3900.025.64593313
CAATGCG6250.025.38629519
TGCGCGA4000.025.00352312
TGGTCGT8700.024.99056251
ACGTGCG4150.024.9266289
ATCGTCA6850.023.65946810
CGATAAT9200.023.63634767
GTACTAG8850.023.3512761
GTCGTCT9500.022.88609353
Produced by FastQC (version 0.11.5)