FastQCFastQC Report
Sat 18 Jun 2016
SRR3137978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989000
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG31950.10689193710270994RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.042.4004171
GTATCAA68100.035.8777431
CGACGAG4600.034.4991324
GTTCGCG501.862955E-434.497416
TATGCCG15200.029.05287243
CAATGCG5750.028.7978319
ATCAACG86350.027.8488733
CGCGACA500.007737817727.59930624
CTCGTAT15400.027.55588339
CAACGCA87800.027.4670855
TATCAAC89500.027.1390152
GCTCGAT6100.026.58218864
TGCGACG6150.026.3651922
TCGTATG17250.026.20065540
TCTCGTA16000.026.09127838
TCAACGC93650.025.788154
AACGCAG95850.025.7357376
ATCGTCA6450.025.67248310
ATCTCGT17000.024.75902737
ATGCCGT17900.024.47785644
Produced by FastQC (version 0.11.5)