FastQCFastQC Report
Sat 18 Jun 2016
SRR3137977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266665
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132070.30953918341374353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.039.1842081
GTATCAA76400.035.1616251
ATCAACG94250.027.8491953
CAACGCA97200.027.2622855
TCAACGC97600.027.0064754
TATCAAC100600.026.2578982
AACGCAG102600.025.96366
TGCGACG4550.024.26022722
ACGCAGA120250.021.8746247
GCTCGAT6300.021.36786864
CGCAGAG124400.021.0627358
ATGCGAC6400.019.9414921
AGAGTAC126300.019.79419311
CGACGAG6000.019.54809224
GCGCGAT2303.4742698E-1019.49282613
TATACCG2303.5106495E-1019.4768035
GTACATG122300.019.2899821
GAGTACT72500.019.07370412
GTACTAG12400.018.8866481
CAGAGTA134900.018.86282510
Produced by FastQC (version 0.11.5)