FastQCFastQC Report
Sat 18 Jun 2016
SRR3137977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266665
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40100.040.024331
GTATCAA78700.032.0157431
TATGCCG14650.027.7889143
CTCGTAT14000.027.35343739
ATCAACG94450.026.4100113
CAACGCA96400.025.9473615
TCTCGTA14450.025.78503838
TCAACGC98300.025.5862274
TATCAAC99100.025.2410132
AACGCAG100250.025.0532446
TCGTATG16600.024.73180640
ATGCCGT17100.023.80745944
TATCTCG16900.022.6591236
TGCCGTC18250.022.11821745
ATCTCGT17700.021.63523337
ACGCAGA119350.020.8699577
CGCAGAG121450.020.395478
GCCGTCT19550.020.11778346
CCGTCTT19900.019.76395247
GTACATG125250.019.6484051
Produced by FastQC (version 0.11.5)