FastQCFastQC Report
Sat 18 Jun 2016
SRR3137976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661434
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136150.2920775023308278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.036.2922331
GTATCAA76700.034.9842071
CAACGCA92750.028.240195
ATCAACG92300.028.1050173
TCAACGC95700.027.2575324
TATCAAC97900.027.0909352
AACGCAG99800.026.5586366
GCTCGAT7600.026.34409164
CTCGATA7850.025.06644465
ACGCAGA113750.022.8206627
TGCGACG5450.022.7841922
CGATAAT8800.022.75318567
GCTCGTC9450.022.64160239
CGCAGAG118250.021.7490888
TCGTCTA10050.021.63627854
GTCGTCT9750.021.59517153
AGAGTAC116950.020.6980311
GTACATG124150.020.3370111
TACATGG123600.019.7580322
TGGTCGT10550.019.63002651
Produced by FastQC (version 0.11.5)