FastQCFastQC Report
Sat 18 Jun 2016
SRR3137976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661434
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.036.820631
GTATCAA77200.032.2389141
TATGCCG14900.026.39631843
CTCGTAT14250.026.38925439
ATCAACG94450.025.899283
CAACGCA94850.025.644285
TCGTATG15700.025.05074740
TCAACGC98100.025.0057164
TATCAAC101000.024.562332
TCTCGTA15500.024.48340438
AACGCAG103650.023.899016
CTCGATA10100.023.56910965
GCTCGAT10300.023.44640464
TGGTCGT10500.023.00054751
GCTCGTC10900.022.4722939
TGCGACG6700.022.1411322
TATCTCG17550.022.01667436
GTCGTCT11000.021.95506953
CGATAAT11050.021.85478667
CGTCATT11300.021.67727526
Produced by FastQC (version 0.11.5)