FastQCFastQC Report
Sat 18 Jun 2016
SRR3137975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611165
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162550.4501317441878175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37250.039.2027441
GTATCAA75200.034.532691
ATCAACG89700.028.2656273
CAACGCA93350.027.3190355
TCAACGC93550.027.2914094
TATCAAC95400.026.7892
AACGCAG99000.026.042386
TGCGACG3700.022.3733622
ACGCAGA112550.022.3605277
CGCAGAG115200.021.6681028
GTACATG104050.020.2575191
AGAGTAC117050.020.12215611
TACATGG105400.019.3129162
GCAGAGT126750.019.1231869
CTCGATA5250.019.06690465
ACATGGG104000.018.9799273
CAGAGTA126000.018.9646910
GAGTACT75700.018.9058212
GCTCGAT4950.018.82834464
CGACGAG4300.018.45063224
Produced by FastQC (version 0.11.5)