FastQCFastQC Report
Sat 18 Jun 2016
SRR3137975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611165
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37700.037.9944761
GTATCAA70550.031.8491331
GCGAATC16800.027.31338733
AATCTCG13650.027.04408636
AATCGCG2400.025.87512636
TATGCCG11650.025.76478843
CTCGTAT11750.025.54515839
CGAATCT15150.025.2780534
ATCAACG87200.024.9259263
TATCAAC90450.024.4893782
TCTCGTA12700.024.17728238
CAACGCA90400.024.1199195
ATCTCGT15300.023.90207537
TCAACGC91750.023.8402274
TCGTATG12250.023.65758340
GGCGAAT21350.023.10847932
AACGCAG96550.022.9045076
TAAGGCG22650.022.23913429
GCTCGAT5150.021.43699864
AGGCGAA24200.021.10015131
Produced by FastQC (version 0.11.5)