FastQCFastQC Report
Sat 18 Jun 2016
SRR3137974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4888534
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182180.37266796139701597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43700.038.150741
GTATCAA84000.032.8057331
ATCAACG96850.027.4653873
TCAACGC98850.027.0527234
CAACGCA99550.026.9366995
TATCAAC104650.025.8078292
AACGCAG105850.025.4665956
ACGCAGA120700.022.0246457
CGCAGAG124150.021.2757038
AGAGTAC126400.019.67237911
GAGTACT79350.018.6464812
GTACATG121900.018.6217611
GCAGAGT142100.018.272899
CAGAGTA137650.018.26332910
TACATGG121150.017.9139672
CGACGAG4450.017.8300424
TGCGACG4100.017.66950622
ACATGGG120700.017.2994943
GTATAGG15050.017.165791
AGTACTT87550.016.90090213
Produced by FastQC (version 0.11.5)