FastQCFastQC Report
Sat 18 Jun 2016
SRR3137974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4888534
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40850.037.931611
GTATCAA80450.032.9444431
TATGCCG20400.028.5819943
CTCGTAT21350.027.14803539
ATCAACG97250.026.854133
TCGTATG21750.026.80738440
TCTCGTA21750.026.64794538
TATCTCG21850.026.21046836
TATCAAC101500.026.002142
CAACGCA100450.025.9645675
TCAACGC101250.025.7591514
AACGCAG106600.024.5955626
ATCTCGT23550.024.31841737
ATGCCGT24500.023.93970144
GCCGTCT24600.023.56164646
CCGTCTT25250.023.36502347
CGTATGC25250.023.09174741
TGCCGTC25400.023.0912145
ACGCAGA123400.020.9109367
CGACGAG4650.020.77372224
Produced by FastQC (version 0.11.5)