FastQCFastQC Report
Sat 18 Jun 2016
SRR3137973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793125
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169690.4473620036249794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.040.045841
GTATCAA73750.033.9957961
CGCGTAA605.4371235E-428.73859810
ATCAACG88750.027.6349873
CGACGAG6300.027.37841824
CAACGCA90950.027.0958865
CAATGCG6350.026.61641519
TATCAAC94150.026.3736842
AACGCAG97250.025.8054056
TCAACGC96150.025.730884
TGCGACG7200.023.95516822
GCTCGAT8150.022.87069164
ACGCAGA108800.022.4684097
CTCGATA8300.022.45766465
ACCGTCG5850.022.40729123
TGGTCGT8500.022.331851
GTGATCG5900.022.2074178
CGCAGAG110350.021.9971688
TATCTCG7250.021.8867623
CGTACGA5700.021.79523860
Produced by FastQC (version 0.11.5)