FastQCFastQC Report
Sat 18 Jun 2016
SRR3137973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793125
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.041.2449261
GTATCAA73700.034.7949681
CGACGAG5400.030.6657524
CTCGTAT16950.028.49547839
TATGCCG17450.028.27285443
ATCAACG91050.027.6244623
CTCGATA7600.027.23710865
CAACGCA92600.027.1982485
GCTCGAT7450.026.85932264
TCGTATG18800.026.79246140
TATCAAC96200.026.5042532
ATCTCGT18900.026.10308537
TCAACGC98950.025.59234
TCTCGTA18750.025.5759138
CAATGCG6500.025.47515719
AACGCAG99750.025.4202966
TGCGACG6600.024.56712522
TATCTCG20750.023.9417736
CATTGCG8850.023.78020729
ATGCCGT20900.023.60580444
Produced by FastQC (version 0.11.5)