FastQCFastQC Report
Sat 18 Jun 2016
SRR3137972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876132
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202110.5214218710817898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.038.840151
GTATCAA68750.031.955781
ATCAACG82650.026.0032333
CAACGCA83550.025.8162465
TCAACGC85700.025.163384
TATCAAC88200.024.5561942
AACGCAG90300.024.0446856
TGCGACG3200.023.71334822
CGACGAG3950.021.83103624
ACGCAGA103500.020.5505497
CAATGCG3900.020.34125919
CGCAGAG107350.019.7510878
GTACATG99500.019.7266451
ATGCGAC4450.019.3778621
TACATGG98650.019.1625942
GCGAACG900.00575855619.1541239
AGAGTAC107950.018.71997511
ACATGGG97900.018.5049823
GAGTACT69000.018.39200412
TATACCG2651.1823431E-1018.2031865
Produced by FastQC (version 0.11.5)