FastQCFastQC Report
Sat 18 Jun 2016
SRR3137972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876132
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG51210.13211624371925412RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.039.7638781
GTATCAA67650.034.647861
TATGCCG21350.029.24883343
CTCGTAT20900.028.72232839
ATCAACG81850.028.11483
TCGTATG22800.027.99326340
CAACGCA83300.027.583995
TATCTCG22300.027.22819536
TCTCGTA22450.026.892638
TCAACGC86150.026.7916054
TATCAAC87250.026.6525732
AACGCAG89400.026.0102586
CGTATGC24850.025.26811641
ATGCCGT24800.024.90171244
CCGTCTT25750.024.11699347
ATCTCGT24950.024.05967537
TGCCGTC25750.023.9830145
GCCGTCT26950.023.29917346
ACGCAGA100300.022.5289127
CGCAGAG104300.021.4664528
Produced by FastQC (version 0.11.5)