FastQCFastQC Report
Sat 18 Jun 2016
SRR3137971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266448
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132930.31157065549609414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.040.2485281
GTATCAA88800.037.0379371
ATCAACG111500.029.0131573
CAACGCA114100.028.6647435
TATCAAC116550.027.9235612
TCAACGC116300.027.7896754
GCTCGAT8100.027.70001264
AACGCAG123200.026.6345086
CTCGATA9000.025.31593365
CGACGAG7050.024.95202624
TGCGACG6800.024.85547822
ACGCAGA140500.022.8454137
CGTACGA4100.022.72686860
GCTCGTC10900.022.47763639
CGCAGAG143650.022.1300078
CGATAAT10600.021.4956767
TGGTCGT11450.021.40170151
GTACTAG11800.021.3057691
CAATGCG7650.021.19072719
CGCACTA8150.020.74880258
Produced by FastQC (version 0.11.5)