FastQCFastQC Report
Sat 18 Jun 2016
SRR3137971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266448
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43700.040.04491
GTATCAA88550.035.0812151
ATCAACG111850.027.5453173
CAACGCA113600.027.1513565
TATCAAC116400.026.5587542
TCAACGC116900.026.3258674
TATGCCG14350.026.20606443
GCTCGAT8100.025.98166564
AACGCAG121450.025.7360956
CTCGTAT15300.024.57831639
CGACGAG6250.023.18334624
CTCGATA8950.023.12865365
TCGTATG16450.023.06979840
TATCTCG17200.022.66532336
CGTCATT9600.022.28140626
ACGATAG4650.022.25769862
TCTCGTA16900.022.25111438
TGCGACG6700.022.14038822
CAATGCG6750.021.97612819
GCTCGTC9650.021.80818739
Produced by FastQC (version 0.11.5)