FastQCFastQC Report
Sat 18 Jun 2016
SRR3137970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619905
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133530.5096749691305601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.039.565931
GTATCAA52000.036.021111
ATCAACG64100.028.4787223
CAACGCA65850.028.0496625
TGCGACG4600.026.99627722
TATCAAC69300.026.7307132
TCAACGC69750.026.177854
AACGCAG71650.025.9258356
CTCGATA4400.025.10475565
GTACATG95750.024.6687341
ATGCGAC5750.024.59283821
TACATGG95550.023.9996592
CGACGAG5450.023.41878924
ACATGGG98000.022.6692033
ACCGTCG5350.022.56611623
GAGTACA63350.022.3386381
CAATGCG5700.021.78146419
GGCGTCG5750.021.5866858
ACGCAGA85900.021.4723057
ATACGGG6100.021.4563773
Produced by FastQC (version 0.11.5)