FastQCFastQC Report
Sat 18 Jun 2016
SRR3137970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619905
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.11072920582998239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.045.1682131
GTATCAA53350.038.4878651
CTCGATA6300.031.76235265
TCGTCTA6750.030.66651554
CGATAAT6900.028.99985967
ATCAACG71500.028.4694773
GCTCGAT7100.028.18349664
CAACGCA72950.027.8563045
TATCAAC73150.027.8278392
TGGTCGT7450.027.32201251
CGTCATT7950.026.9060426
TCAACGC76350.026.7513794
AACGCAG77650.026.5690886
TCGATAA8000.025.44410966
GTCGTCT8000.025.44362353
TGCGACG4250.025.16362222
TAGGTCG8500.024.35235621
CGTCTAC8600.024.06964755
GTACTAG7600.023.1578311
CATTGCG9350.022.87732929
Produced by FastQC (version 0.11.5)