FastQCFastQC Report
Sat 18 Jun 2016
SRR3137969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445222
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162060.3645712182653644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35850.035.4441571
GTATCAA68750.031.8059581
ATCAACG76800.027.1379933
CAACGCA79600.026.4537945
TATCAAC83100.025.5679822
AACGCAG84100.025.4936836
TCAACGC81650.025.4895554
CGACGAG4650.023.73905824
TGCGACG4750.023.2371922
CAATGCG5000.022.76133319
ACGCAGA96750.021.700917
CGCAGAG96650.021.4748828
AGAGTAC104300.019.14294811
ATGCGAC6150.019.0667721
GAGTACT64750.018.2140212
TACGCCG950.0078858218.1387045
CAGAGTA110550.018.09045410
TACATGG97000.017.9635072
GTACATG101650.017.8868541
GCAGAGT112700.017.8663129
Produced by FastQC (version 0.11.5)