FastQCFastQC Report
Sat 18 Jun 2016
SRR3137969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445222
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.034.9871941
GTATCAA63350.033.1237341
CTCGTAT12100.031.9343939
TCGTATG15200.029.05309140
TATGCCG15400.028.2277243
ATCAACG72100.028.088833
AATCTCG14300.027.7445736
TCTCGTA14100.027.64906138
CAACGCA74150.027.2192125
TCAACGC76400.026.5982344
TATCAAC78150.026.4885392
ATGCCGT16750.025.95264844
AACGCAG80150.025.4823386
TGCCGTC18250.024.38668645
CGTATGC18200.024.26384741
CCGTCTT18800.023.67324847
GCCGTCT18700.023.61534746
ATCTCGT17100.023.201637
CGACGAG4800.022.99984724
TACGCGC1352.6629823E-722.99984758
Produced by FastQC (version 0.11.5)