FastQCFastQC Report
Sat 18 Jun 2016
SRR3137968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184746
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172370.41190074618626793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34750.039.536561
GTATCAA69400.032.746531
ATCAACG84200.026.3454323
CAACGCA86600.025.944695
TCAACGC87650.025.3931264
TATCAAC89300.024.9478762
AACGCAG92300.024.4956266
CGACGAG5000.023.45687524
TGCGACG5050.021.85794822
CGCACTA5300.021.4880658
CAATGCG5200.021.22437519
ACGCAGA106850.020.8099237
ATGCGAC6150.020.75059321
CGCAGAG108650.020.3406898
AGAGTAC110000.019.02880111
CTCGATA5600.018.49409365
GTACATG108950.018.4572261
GAGTACT63250.018.3736412
GTGTAGC22900.018.344471
TACATGG108750.018.079552
Produced by FastQC (version 0.11.5)