FastQCFastQC Report
Sat 18 Jun 2016
SRR3137968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184746
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.037.758391
GTATCAA66950.035.2618681
ATCAACG82100.028.0302353
CAACGCA84550.027.0955875
TCAACGC87050.026.4759624
TATCAAC88600.026.2470572
AACGCAG91100.025.3731616
TATGCCG16600.022.65329243
CTCGTAT13800.021.4999539
ACGCAGA105450.021.4609747
TCGTATG17200.020.45925740
CGCAGAG109250.020.3987378
CTCGCGC2201.9099389E-1020.38680560
TCGACGA3600.020.12519651
ATGCCGT18350.019.92887344
GTACATG108500.019.817921
AATCTCG15150.019.81160236
AGAGTAC111450.019.43843711
CTCGATA5550.019.27045865
TCTCGTA15000.019.09018538
Produced by FastQC (version 0.11.5)