FastQCFastQC Report
Sat 18 Jun 2016
SRR3137967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4043914
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235130.581441642923168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.039.596121
GTATCAA71700.034.9202041
CAACGCA85850.028.4213685
ATCAACG85400.028.2767333
AACGCAG91050.027.0674936
TCAACGC89600.027.0335124
TATCAAC92150.026.5340182
ACGCAGA103400.023.2733637
CGCAGAG105850.022.6380868
TGCGACG4450.021.70227822
GAGTACT69050.021.57666412
AGAGTAC107900.021.28527811
CAGAGTA117600.019.85044310
GCTCGAT6100.019.80595264
AGTACTT76100.019.7142213
CGACGAG5100.019.61308524
GCAGAGT120350.019.5389049
GTACATG100250.019.2038781
CTCGATA6400.018.87895465
TACATGG98600.018.6860242
Produced by FastQC (version 0.11.5)