FastQCFastQC Report
Sat 18 Jun 2016
SRR3137967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4043914
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45900.11350389746171655RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.039.4609641
GTATCAA73350.035.621811
TATGCCG18950.031.31493443
CTCGTAT16300.030.26666539
AATCTCG17200.029.48453536
TCTCGTA17250.028.79944838
ATCAACG89400.028.3656563
CAACGCA89850.028.108395
TCGTATG20750.028.09860240
ATGCCGT20500.027.93743144
TCAACGC93650.027.2625794
TATCAAC94250.027.12632
AACGCAG97450.026.0929556
CCGTCTT22550.025.85601847
ATCTCGT19750.025.85266937
TGCCGTC22450.025.81815345
CGTATGC23700.024.89284941
GCCGTCT23200.024.68578746
CTCGATA5350.024.50389965
GCTCGAT5650.023.81340864
Produced by FastQC (version 0.11.5)