FastQCFastQC Report
Sat 18 Jun 2016
SRR3137966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4966156
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218510.4399982602238029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.037.4138951
GTATCAA81950.032.5722661
TGCGACG8100.028.10454222
CAATGCG8550.027.43107619
CGACGAG8450.027.34974124
ATCAACG96000.026.9476473
CAACGCA98000.026.5146455
TATCAAC103100.025.6552262
TCAACGC101750.025.6331064
GCTCGAT8850.025.35098364
AACGCAG106600.024.8326726
CTCGATA10350.021.67780365
GTACTAG15900.021.6619781
ATGCGAC11200.021.55680721
ACGCAGA120100.021.472537
ATCGTCA10800.021.39323610
CGCAGAG124250.020.5074738
GTGTAGC23900.020.1755491
GTACATG137850.019.9134641
CGGGATA7900.019.65941650
Produced by FastQC (version 0.11.5)