FastQCFastQC Report
Sat 18 Jun 2016
SRR3137966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4966156
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.038.2111971
GTATCAA82050.033.6523361
GCTCGAT9300.029.30622564
TATGCCG20050.028.39158843
CTCGATA10000.027.2547965
ATCAACG98850.027.0148093
CAACGCA99500.026.838335
TATCAAC104750.026.1198022
TCAACGC103750.025.9384564
CTCGTAT17700.025.53395739
TGGTCGT11100.025.48705951
TGCGACG6950.025.31558822
AACGCAG107850.025.2075466
AATCTCG18900.024.64266836
CGATAAT11400.024.21082567
TCTCGTA19350.023.3564138
CGACGAG7700.023.29805224
TCGTATG23550.023.14655140
TCGTCTA12400.023.0932654
GCTCGTC12350.022.62758439
Produced by FastQC (version 0.11.5)