FastQCFastQC Report
Sat 18 Jun 2016
SRR3137965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4737916
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275550.5815848149270693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.038.1106151
GTATCAA79150.035.0698931
ATCAACG94400.028.207423
CAACGCA95500.028.14875
TCAACGC98350.027.1175884
TATCAAC100050.026.8848232
AACGCAG101550.026.815056
CTCGATA6650.022.84004265
ACGCAGA116800.022.6411677
CGCAGAG119150.022.022398
GCTCGAT7050.021.0542964
ACGTGCG3050.020.3495949
AGAGTAC120450.020.29892311
GTACATG122650.019.8799841
GAGTACT71650.019.78212712
TACATGG121350.019.554772
ACATGGG122700.018.8660323
CAGAGTA132050.018.80163210
GCAGAGT137400.018.4954059
CGTACGA4750.018.16552260
Produced by FastQC (version 0.11.5)