FastQCFastQC Report
Sat 18 Jun 2016
SRR3137965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4737916
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58220.12288103039395379RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37700.038.4515271
GTATCAA74400.033.4942251
TATGCCG24500.032.24751743
TCGTATG26300.029.6469240
AATCTCG20750.029.59539836
CTCGTAT20400.029.42699439
TCTCGTA21100.028.45044538
ATCTCGT21850.027.94757537
ATGCCGT29050.026.60288644
ATCAACG91950.026.4151083
CAACGCA92550.026.280585
TGCCGTC29950.026.26424245
CGTATGC30350.026.2591241
CCGTCTT28350.026.04279547
TCAACGC95850.025.5200184
TATCAAC96900.025.3154952
AACGCAG99950.024.6107256
CGTACGA5550.024.24342260
GCCGTCT30300.024.02518346
TGCGACG6100.023.7532722
Produced by FastQC (version 0.11.5)