FastQCFastQC Report
Sat 18 Jun 2016
SRR3137964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853182
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148020.38415003495812033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.039.057291
GTATCAA74250.034.146771
CAATGCG6950.028.78004519
CAACGCA89250.028.6164255
ATCAACG88800.028.3238013
GCTCGAT7150.028.00196364
TCAACGC91650.027.5239374
AACGCAG95150.027.0635626
TATCAAC93050.027.0625842
CGACGAG7400.027.03377324
TGCGACG7200.026.82452622
GTACTAG10400.025.8362541
CTCGATA7500.025.3134365
ATGCGAC8200.025.2349621
TGGTCGT8700.023.80232451
CGCACTA8100.023.4359258
ACGCAGA108750.023.112097
CGATAAT9250.022.7649167
GTCGTCT9150.022.63201353
CGCAGAG112750.022.0186548
Produced by FastQC (version 0.11.5)