FastQCFastQC Report
Sat 18 Jun 2016
SRR3137964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853182
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.038.919241
GTATCAA71250.032.7895971
TGCGACG6800.027.90480422
ATCAACG88000.025.6780053
CGACGAG7150.025.5737924
CAACGCA88600.025.5041165
TATCAAC92750.025.0696722
TCAACGC91350.024.9629334
CAATGCG7800.024.32568719
TATGCCG11300.024.11918343
AATCTCG12600.024.09526636
AACGCAG96000.023.8975336
GCGAATC15100.023.30526533
CTCGTAT12000.022.71252639
ATGCGAC9150.022.62297621
TCTCGTA12550.021.99205638
TCGTATG11950.021.94144640
GTACTAG11800.021.9336831
CGCACTA7650.021.64652358
ATCTCGT13750.021.32729537
Produced by FastQC (version 0.11.5)