FastQCFastQC Report
Sat 18 Jun 2016
SRR3137963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4507233
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283630.6292774302992545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.037.816571
GTATCAA79050.033.2915951
ATCAACG92950.027.6518543
CAACGCA93800.027.5975075
TATCAAC98000.026.57262
TCAACGC97150.026.4614074
AACGCAG100150.026.058546
TGCGACG5150.023.44087222
GCTCGAT5800.023.21068264
ACGCAGA113800.022.4218777
CGCAGAG115550.021.724698
CTCGATA6650.020.76344165
GAGTACT66150.020.43649112
GTACATG124100.020.318071
CAATGCG6000.020.11963319
CGACGAG6050.019.95579724
AGAGTAC119100.019.63172111
GTATAAG16250.019.5019281
TACATGG123500.019.384572
AGTACTT70850.018.88692713
Produced by FastQC (version 0.11.5)