FastQCFastQC Report
Sat 18 Jun 2016
SRR3137963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4507233
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG57250.1270180618574633RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.040.4410441
GTATCAA78100.033.4102821
TATGCCG26250.028.38903843
AATCTCG21950.027.81988536
CTCGTAT21750.027.7584639
ATCAACG94150.027.1173523
CAACGCA95550.026.7558365
TCTCGTA22600.026.56179238
TATCAAC99400.025.9633482
TCGTATG27300.025.9067340
TCAACGC99100.025.727754
AACGCAG101500.025.4927466
ATCTCGT23850.025.45897137
ATGCCGT30450.024.36000644
CGTATGC31100.023.5178141
TGCCGTC31850.023.5058845
CCGTCTT31850.023.39755847
GTATGCC34000.022.32364842
GCCGTCT33000.021.85035946
ACGCAGA116250.021.5444877
Produced by FastQC (version 0.11.5)